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cd51-pe  (Thermo Fisher)


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    Thermo Fisher cd51-pe
    Cd51 Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd51-pe/cd51+rmv+7/pmc10083278__DataSheet_1-2-0-2
    Average 90 stars, based on 1 article reviews
    cd51-pe - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Regionally Restricted Hox Function in Adult Bone Marrow-Multipotent Mesenchymal Stem/Stromal Cells
    Article Snippet: For non-endothelial stroma and MSC exclusion; PDGFRα+/CD140a-APC (ebioscience, Clone APA5, 1:100) or PDGFRα/CD140a-PECF594 (BD, clone APA5, 1:100), CD51-PE (ebioscience, Clone RMV-7, 1:100), Biotinylated-goat-anti-Leptin Receptor (R&D, Cat. BAF497, 1:200), and Streptavidin-Qdot565 (Invitrogen, Cat. Q10133MP, 1:500) or BV605 (Biolegend, 405229, 1:500).

    Article Title: Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma
    Article Snippet: For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.

    Article Title: Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma
    Article Snippet: The following antibodies were used: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD3e-PE/Dazzle594 (Biolegend, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD31-PE/CF594 (BD, clone MEC13.3), CD44-BV510 (Biolegend, clone IM7), CD45-APC/Cy7 (eBioscience, clone 30-F11), CD51-PE (eBioscience, clone RMY-7), CD69-AF647 (Biolegend, clone H1.2F3), Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend, clone 29F.1A12), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Tim3-PE/Cy7 (Biolegend, clone RMT3-23), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4), Lsel-AF700 (Biolegend, clone MEL-14), Sca-1-PerCP/Cy5.5 (Invitrogen, clone D7), Ter119-BV510 (Biolegend, clone TER-119), Streptavidin-PE/Cy7 (Biolegend).

    Article Title: SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism
    Article Snippet: The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).

    Article Title: Supplementary Materials for Shao J et al., Effects of aging and macrophages on stem Leydig cells proliferation and differentiation in vitro Supplementary Table S1: Antibodies information Primary Antibody
    Article Snippet: CD51-PE 1:40 eBioscience, San Diego, CA 2087658 F4/80-APC 1:20 MultiSciences, Hangzhou, CHN 70-AM048005-100 CYP17A1 1:200 Cell Signaling Technology, MA, Boston, USA 94004 HSD3B1 1:500 Novus Biologicals,Littleton, Colorado, USA NB110-78644 CD51 1:500 Abcam, Cambridge, UK ab179475 F4/80 1:500 Abcam, Cambridge, UK ab16911 Dylight 488 conjugated of goat anti-Rat IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN 060702 Dylight 594 conjugated of goat anti-Rabbit IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN A00743 Goat anti-Rabbit IgG-AlexaFlour 488 IF(1:500) Absin,Shanghai,CHN abs20025

    Isolation:

    Article Title: Regionally Restricted Hox Function in Adult Bone Marrow-Multipotent Mesenchymal Stem/Stromal Cells
    Article Snippet: For non-endothelial stroma and MSC exclusion; PDGFRα+/CD140a-APC (ebioscience, Clone APA5, 1:100) or PDGFRα/CD140a-PECF594 (BD, clone APA5, 1:100), CD51-PE (ebioscience, Clone RMV-7, 1:100), Biotinylated-goat-anti-Leptin Receptor (R&D, Cat. BAF497, 1:200), and Streptavidin-Qdot565 (Invitrogen, Cat. Q10133MP, 1:500) or BV605 (Biolegend, 405229, 1:500).

    Article Title: Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma
    Article Snippet: For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.

    Article Title: Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma
    Article Snippet: The following antibodies were used: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD3e-PE/Dazzle594 (Biolegend, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD31-PE/CF594 (BD, clone MEC13.3), CD44-BV510 (Biolegend, clone IM7), CD45-APC/Cy7 (eBioscience, clone 30-F11), CD51-PE (eBioscience, clone RMY-7), CD69-AF647 (Biolegend, clone H1.2F3), Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend, clone 29F.1A12), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Tim3-PE/Cy7 (Biolegend, clone RMT3-23), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4), Lsel-AF700 (Biolegend, clone MEL-14), Sca-1-PerCP/Cy5.5 (Invitrogen, clone D7), Ter119-BV510 (Biolegend, clone TER-119), Streptavidin-PE/Cy7 (Biolegend).

    Article Title: SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism
    Article Snippet: The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).

    Article Title: Supplementary Materials for Shao J et al., Effects of aging and macrophages on stem Leydig cells proliferation and differentiation in vitro Supplementary Table S1: Antibodies information Primary Antibody
    Article Snippet: CD51-PE 1:40 eBioscience, San Diego, CA 2087658 F4/80-APC 1:20 MultiSciences, Hangzhou, CHN 70-AM048005-100 CYP17A1 1:200 Cell Signaling Technology, MA, Boston, USA 94004 HSD3B1 1:500 Novus Biologicals,Littleton, Colorado, USA NB110-78644 CD51 1:500 Abcam, Cambridge, UK ab179475 F4/80 1:500 Abcam, Cambridge, UK ab16911 Dylight 488 conjugated of goat anti-Rat IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN 060702 Dylight 594 conjugated of goat anti-Rabbit IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN A00743 Goat anti-Rabbit IgG-AlexaFlour 488 IF(1:500) Absin,Shanghai,CHN abs20025

    Expressing:

    Article Title: Regionally Restricted Hox Function in Adult Bone Marrow-Multipotent Mesenchymal Stem/Stromal Cells
    Article Snippet: For non-endothelial stroma and MSC exclusion; PDGFRα+/CD140a-APC (ebioscience, Clone APA5, 1:100) or PDGFRα/CD140a-PECF594 (BD, clone APA5, 1:100), CD51-PE (ebioscience, Clone RMV-7, 1:100), Biotinylated-goat-anti-Leptin Receptor (R&D, Cat. BAF497, 1:200), and Streptavidin-Qdot565 (Invitrogen, Cat. Q10133MP, 1:500) or BV605 (Biolegend, 405229, 1:500).

    Article Title: Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma
    Article Snippet: For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.

    Article Title: Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma
    Article Snippet: The following antibodies were used: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD3e-PE/Dazzle594 (Biolegend, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD31-PE/CF594 (BD, clone MEC13.3), CD44-BV510 (Biolegend, clone IM7), CD45-APC/Cy7 (eBioscience, clone 30-F11), CD51-PE (eBioscience, clone RMY-7), CD69-AF647 (Biolegend, clone H1.2F3), Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend, clone 29F.1A12), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Tim3-PE/Cy7 (Biolegend, clone RMT3-23), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4), Lsel-AF700 (Biolegend, clone MEL-14), Sca-1-PerCP/Cy5.5 (Invitrogen, clone D7), Ter119-BV510 (Biolegend, clone TER-119), Streptavidin-PE/Cy7 (Biolegend).

    Article Title: SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism
    Article Snippet: The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).

    Article Title: Supplementary Materials for Shao J et al., Effects of aging and macrophages on stem Leydig cells proliferation and differentiation in vitro Supplementary Table S1: Antibodies information Primary Antibody
    Article Snippet: CD51-PE 1:40 eBioscience, San Diego, CA 2087658 F4/80-APC 1:20 MultiSciences, Hangzhou, CHN 70-AM048005-100 CYP17A1 1:200 Cell Signaling Technology, MA, Boston, USA 94004 HSD3B1 1:500 Novus Biologicals,Littleton, Colorado, USA NB110-78644 CD51 1:500 Abcam, Cambridge, UK ab179475 F4/80 1:500 Abcam, Cambridge, UK ab16911 Dylight 488 conjugated of goat anti-Rat IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN 060702 Dylight 594 conjugated of goat anti-Rabbit IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN A00743 Goat anti-Rabbit IgG-AlexaFlour 488 IF(1:500) Absin,Shanghai,CHN abs20025

    Knock-Out:

    Article Title: Regionally Restricted Hox Function in Adult Bone Marrow-Multipotent Mesenchymal Stem/Stromal Cells
    Article Snippet: For non-endothelial stroma and MSC exclusion; PDGFRα+/CD140a-APC (ebioscience, Clone APA5, 1:100) or PDGFRα/CD140a-PECF594 (BD, clone APA5, 1:100), CD51-PE (ebioscience, Clone RMV-7, 1:100), Biotinylated-goat-anti-Leptin Receptor (R&D, Cat. BAF497, 1:200), and Streptavidin-Qdot565 (Invitrogen, Cat. Q10133MP, 1:500) or BV605 (Biolegend, 405229, 1:500).

    Article Title: Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma
    Article Snippet: For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.

    Article Title: Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma
    Article Snippet: The following antibodies were used: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD3e-PE/Dazzle594 (Biolegend, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD31-PE/CF594 (BD, clone MEC13.3), CD44-BV510 (Biolegend, clone IM7), CD45-APC/Cy7 (eBioscience, clone 30-F11), CD51-PE (eBioscience, clone RMY-7), CD69-AF647 (Biolegend, clone H1.2F3), Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend, clone 29F.1A12), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Tim3-PE/Cy7 (Biolegend, clone RMT3-23), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4), Lsel-AF700 (Biolegend, clone MEL-14), Sca-1-PerCP/Cy5.5 (Invitrogen, clone D7), Ter119-BV510 (Biolegend, clone TER-119), Streptavidin-PE/Cy7 (Biolegend).

    Article Title: SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism
    Article Snippet: The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).

    Article Title: Supplementary Materials for Shao J et al., Effects of aging and macrophages on stem Leydig cells proliferation and differentiation in vitro Supplementary Table S1: Antibodies information Primary Antibody
    Article Snippet: CD51-PE 1:40 eBioscience, San Diego, CA 2087658 F4/80-APC 1:20 MultiSciences, Hangzhou, CHN 70-AM048005-100 CYP17A1 1:200 Cell Signaling Technology, MA, Boston, USA 94004 HSD3B1 1:500 Novus Biologicals,Littleton, Colorado, USA NB110-78644 CD51 1:500 Abcam, Cambridge, UK ab179475 F4/80 1:500 Abcam, Cambridge, UK ab16911 Dylight 488 conjugated of goat anti-Rat IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN 060702 Dylight 594 conjugated of goat anti-Rabbit IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN A00743 Goat anti-Rabbit IgG-AlexaFlour 488 IF(1:500) Absin,Shanghai,CHN abs20025

    Transplantation Assay:

    Article Title: Regionally Restricted Hox Function in Adult Bone Marrow-Multipotent Mesenchymal Stem/Stromal Cells
    Article Snippet: For non-endothelial stroma and MSC exclusion; PDGFRα+/CD140a-APC (ebioscience, Clone APA5, 1:100) or PDGFRα/CD140a-PECF594 (BD, clone APA5, 1:100), CD51-PE (ebioscience, Clone RMV-7, 1:100), Biotinylated-goat-anti-Leptin Receptor (R&D, Cat. BAF497, 1:200), and Streptavidin-Qdot565 (Invitrogen, Cat. Q10133MP, 1:500) or BV605 (Biolegend, 405229, 1:500).

    Article Title: Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma
    Article Snippet: For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.

    Article Title: Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma
    Article Snippet: The following antibodies were used: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD3e-PE/Dazzle594 (Biolegend, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD31-PE/CF594 (BD, clone MEC13.3), CD44-BV510 (Biolegend, clone IM7), CD45-APC/Cy7 (eBioscience, clone 30-F11), CD51-PE (eBioscience, clone RMY-7), CD69-AF647 (Biolegend, clone H1.2F3), Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend, clone 29F.1A12), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Tim3-PE/Cy7 (Biolegend, clone RMT3-23), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4), Lsel-AF700 (Biolegend, clone MEL-14), Sca-1-PerCP/Cy5.5 (Invitrogen, clone D7), Ter119-BV510 (Biolegend, clone TER-119), Streptavidin-PE/Cy7 (Biolegend).

    Article Title: SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism
    Article Snippet: The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).

    Article Title: Supplementary Materials for Shao J et al., Effects of aging and macrophages on stem Leydig cells proliferation and differentiation in vitro Supplementary Table S1: Antibodies information Primary Antibody
    Article Snippet: CD51-PE 1:40 eBioscience, San Diego, CA 2087658 F4/80-APC 1:20 MultiSciences, Hangzhou, CHN 70-AM048005-100 CYP17A1 1:200 Cell Signaling Technology, MA, Boston, USA 94004 HSD3B1 1:500 Novus Biologicals,Littleton, Colorado, USA NB110-78644 CD51 1:500 Abcam, Cambridge, UK ab179475 F4/80 1:500 Abcam, Cambridge, UK ab16911 Dylight 488 conjugated of goat anti-Rat IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN 060702 Dylight 594 conjugated of goat anti-Rabbit IgG (H+L) IF(1:400) MultiSciences, Hangzhou, CHN A00743 Goat anti-Rabbit IgG-AlexaFlour 488 IF(1:500) Absin,Shanghai,CHN abs20025



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    itgav (cd51) pe  (Becton Dickinson)
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    Becton Dickinson itgav (cd51) pe
    ( a ) APL and AML mice model showing splenomegaly compared to control as well as osteoblast percentage (CD45 − Sca1 − <t>CD51</t> + ) cells in bone marrow compared to control mice APL (n = 12) AML (n = 3) on day 28. ( b ) Immunoblot analysis with relative quantification of RUNX2 in bone marrow stromal cells expanded from control and leukemic mice (n = 3). ( c ) Workflow and microscopic picture of Alizarin Red S staining (osteoblast differentiation) and Oil Red O staining (adipocyte differentiation) of the expanded stroma after 14 days of differentiation of leukemic MSCs and control MSCs. The scale bar is 50 µm. ( d ) Quantification of Alizarin Red S and Oil Red O staining demonstrated a substantial increase in osteoblast differentiation (Alizarin Red S staining). In contrast, the adipocyte differentiation (Oil Red O) showed an insignificant variation in control MSCs and leukemic MSCs. e) The MSC expanded from control mice and was co-cultured with mice leukemic cell line C1498 for 48 h, followed by induction for 14 days. The scale bar is 50 µm. The co-cultured MSCs showed an increased osteoblast differentiation without significant change in adipocyte differentiation, as demonstrated in the microscopic picture and colorimetric analysis (f). (*— P = 0.05, ns—not significant).
    Itgav (Cd51) Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd51-pe/mouse+anti+human+cd51+61/pmc09631564__advancesADV2021005992___suppl1-206-245-251
    Average 90 stars, based on 1 article reviews
    itgav (cd51) pe - by Bioz Stars, 2026-09
    90/100 stars
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    cd51-pe (rmv-7  (Becton Dickinson)
    90
    Becton Dickinson cd51-pe (rmv-7
    ( a ) APL and AML mice model showing splenomegaly compared to control as well as osteoblast percentage (CD45 − Sca1 − <t>CD51</t> + ) cells in bone marrow compared to control mice APL (n = 12) AML (n = 3) on day 28. ( b ) Immunoblot analysis with relative quantification of RUNX2 in bone marrow stromal cells expanded from control and leukemic mice (n = 3). ( c ) Workflow and microscopic picture of Alizarin Red S staining (osteoblast differentiation) and Oil Red O staining (adipocyte differentiation) of the expanded stroma after 14 days of differentiation of leukemic MSCs and control MSCs. The scale bar is 50 µm. ( d ) Quantification of Alizarin Red S and Oil Red O staining demonstrated a substantial increase in osteoblast differentiation (Alizarin Red S staining). In contrast, the adipocyte differentiation (Oil Red O) showed an insignificant variation in control MSCs and leukemic MSCs. e) The MSC expanded from control mice and was co-cultured with mice leukemic cell line C1498 for 48 h, followed by induction for 14 days. The scale bar is 50 µm. The co-cultured MSCs showed an increased osteoblast differentiation without significant change in adipocyte differentiation, as demonstrated in the microscopic picture and colorimetric analysis (f). (*— P = 0.05, ns—not significant).
    Cd51 Pe (Rmv 7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd51-pe/mouse+anti+human+cd51+61/pmc10655637-222-36-3
    Average 90 stars, based on 1 article reviews
    cd51-pe (rmv-7 - by Bioz Stars, 2026-09
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    anti-cd51 antibody conjugated with pe  (Thermo Fisher)
    90
    Thermo Fisher anti-cd51 antibody conjugated with pe
    ( a ) APL and AML mice model showing splenomegaly compared to control as well as osteoblast percentage (CD45 − Sca1 − <t>CD51</t> + ) cells in bone marrow compared to control mice APL (n = 12) AML (n = 3) on day 28. ( b ) Immunoblot analysis with relative quantification of RUNX2 in bone marrow stromal cells expanded from control and leukemic mice (n = 3). ( c ) Workflow and microscopic picture of Alizarin Red S staining (osteoblast differentiation) and Oil Red O staining (adipocyte differentiation) of the expanded stroma after 14 days of differentiation of leukemic MSCs and control MSCs. The scale bar is 50 µm. ( d ) Quantification of Alizarin Red S and Oil Red O staining demonstrated a substantial increase in osteoblast differentiation (Alizarin Red S staining). In contrast, the adipocyte differentiation (Oil Red O) showed an insignificant variation in control MSCs and leukemic MSCs. e) The MSC expanded from control mice and was co-cultured with mice leukemic cell line C1498 for 48 h, followed by induction for 14 days. The scale bar is 50 µm. The co-cultured MSCs showed an increased osteoblast differentiation without significant change in adipocyte differentiation, as demonstrated in the microscopic picture and colorimetric analysis (f). (*— P = 0.05, ns—not significant).
    Anti Cd51 Antibody Conjugated With Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd51-pe/cd51+rmv+7/pm37697644-329-16-21
    Average 90 stars, based on 1 article reviews
    anti-cd51 antibody conjugated with pe - by Bioz Stars, 2026-09
    90/100 stars
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    Image Search Results


    ( a ) APL and AML mice model showing splenomegaly compared to control as well as osteoblast percentage (CD45 − Sca1 − CD51 + ) cells in bone marrow compared to control mice APL (n = 12) AML (n = 3) on day 28. ( b ) Immunoblot analysis with relative quantification of RUNX2 in bone marrow stromal cells expanded from control and leukemic mice (n = 3). ( c ) Workflow and microscopic picture of Alizarin Red S staining (osteoblast differentiation) and Oil Red O staining (adipocyte differentiation) of the expanded stroma after 14 days of differentiation of leukemic MSCs and control MSCs. The scale bar is 50 µm. ( d ) Quantification of Alizarin Red S and Oil Red O staining demonstrated a substantial increase in osteoblast differentiation (Alizarin Red S staining). In contrast, the adipocyte differentiation (Oil Red O) showed an insignificant variation in control MSCs and leukemic MSCs. e) The MSC expanded from control mice and was co-cultured with mice leukemic cell line C1498 for 48 h, followed by induction for 14 days. The scale bar is 50 µm. The co-cultured MSCs showed an increased osteoblast differentiation without significant change in adipocyte differentiation, as demonstrated in the microscopic picture and colorimetric analysis (f). (*— P = 0.05, ns—not significant).

    Journal: Scientific Reports

    Article Title: Ablation of Wnt signaling in bone marrow stromal cells overcomes microenvironment-mediated drug resistance in acute myeloid leukemia

    doi: 10.1038/s41598-024-58860-8

    Figure Lengend Snippet: ( a ) APL and AML mice model showing splenomegaly compared to control as well as osteoblast percentage (CD45 − Sca1 − CD51 + ) cells in bone marrow compared to control mice APL (n = 12) AML (n = 3) on day 28. ( b ) Immunoblot analysis with relative quantification of RUNX2 in bone marrow stromal cells expanded from control and leukemic mice (n = 3). ( c ) Workflow and microscopic picture of Alizarin Red S staining (osteoblast differentiation) and Oil Red O staining (adipocyte differentiation) of the expanded stroma after 14 days of differentiation of leukemic MSCs and control MSCs. The scale bar is 50 µm. ( d ) Quantification of Alizarin Red S and Oil Red O staining demonstrated a substantial increase in osteoblast differentiation (Alizarin Red S staining). In contrast, the adipocyte differentiation (Oil Red O) showed an insignificant variation in control MSCs and leukemic MSCs. e) The MSC expanded from control mice and was co-cultured with mice leukemic cell line C1498 for 48 h, followed by induction for 14 days. The scale bar is 50 µm. The co-cultured MSCs showed an increased osteoblast differentiation without significant change in adipocyte differentiation, as demonstrated in the microscopic picture and colorimetric analysis (f). (*— P = 0.05, ns—not significant).

    Article Snippet: Flow antibodies such as mouse CD45-pacific blue (30-F11), CD117-PE (2B8), Ly6A/E (Sca-1)-FITC (E13-161.7), CD51-PE (RMV-7), Ly6G/6C (GR1)-APC (RB6-8C5), CD11b (MAC-1)-FITC (M1/70) were acquired from BD Biosciences (New Jersey, USA).

    Techniques: Western Blot, Staining, Cell Culture